Pieter de Tombe, PhD

Cell and Molecular Physiology

Professor
Department Chairperson, Cell and Molecular Physiology

Languages Spoken

English, Dutch, German

Biography

Interests
Photography, Travel

Research

  • A Green Tea Catechin Normalizes the Enhanced Ca2+ Sensitivity of Myofilaments Regulated by a Hypertrophic Cardiomyopathy Associated Mutation in Human Cardiac Troponin I (K206I) Warren, C. M.; Karam, C. N.; Wolska, B. M.; Kobayashi, T.; de Tombe, P. P.; Arteaga, G. M.; Bos, J. M.; Ackerman, M. J.; Solaro, R. J., Circulation.Cardiovascular Genetics
  • Acute Inotropic and Lusitropic Effects of Cardiomyopathic R9C Mutation of Phospholamban Abrol, N.; de Tombe, P. P.; Robia, S. L., The Journal of biological chemistry
  • Cardiac muscle mechanics: Sarcomere length matters de Tombe, P. P.; Ter Keurs, H. E., Journal of Molecular and Cellular Cardiology
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  • Cardiac myosin binding protein C and Troponin-I phosphorylation independently modulate myofilament length dependent activation Kumar, M.; Govindan, S.; Zhang, M.; Khairallah, R.; Martin, J. L.; Sadayappan, S.; de Tombe, P. P., The Journal of biological chemistry
  • Green Tea Catechin Normalizes the Enhanced Ca2+ Sensitivity of Myofilaments Regulated by a Hypertrophic Cardiomyopathy-Associated Mutation in Human Cardiac Troponin I (K206I) Warren, C. M.; Karam, C. N.; Wolska, B. M.; Kobayashi, T.; de Tombe, P. P.; Arteaga, G. M.; Bos, J. M.; Ackerman, M. J.; Solaro, R. J., Circulation.Cardiovascular Genetics
  • Increased Energy Demand during Adrenergic Receptor Stimulation Contributes to Ca(2+) Wave Generation Bovo, E.; Mazurek, S. R.; de Tombe, P. P.; Zima, A. V., Biophysical journal
  • Myosin light chain phosphorylation to the rescue Granzier, H. L.; de Tombe, P. P., Proceedings of the National Academy of Sciences of the United States of America
  • Pacemaker-induced transient asynchrony suppresses heart failure progression Kirk, J. A.; Chakir, K.; Lee, K. H.; Karst, E.; Holewinski, R. J.; Pironti, G.; Tunin, R. S.; Pozios, I.; Abraham, T. P.; de Tombe, P.; Rockman, H. A.; Van Eyk, J. E.; Craig, R.; Farazi, T. G.; Kass, D. A., Science translational medicine
  • Rapid large-scale purification of myofilament proteins using a cleavable His6-tag Zhang, M.; Martin, J. L.; Kumar, M.; Khairallah, R. J.; de Tombe, P. P., American journal of physiology.Heart and circulatory physiology
  • Cardiac myosin binding protein-C as a central target of cardiac sarcomere signaling: a special mini review series Sadayappan, S.; de Tombe, P. P., Pflugers Archiv : European journal of physiology
  • Cardiac resynchronization sensitizes the sarcomere to calcium by reactivating GSK-3beta Kirk, J. A.; Holewinski, R. J.; Kooij, V.; Agnetti, G.; Tunin, R. S.; Witayavanitkul, N.; de Tombe, P. P.; Gao, W. D.; Van Eyk, J.; Kass, D. A., The Journal of clinical investigation
  • Cardiac troponin I tyrosine 26 phosphorylation decreases myofilament Ca sensitivity and accelerates deactivation Salhi, H. E.; Walton, S. D.; Hassel, N. C.; Brundage, E. A.; de Tombe, P. P.; Janssen, P. M.; Davis, J. P.; Biesiadecki, B. J., Journal of Molecular and Cellular Cardiology
  • Cardiomyocyte-specific expression of CRNK the C-terminal domain of PYK2, maintains ventricular function and slows ventricular remodeling in a mouse model of dilated cardiomyopathy, Koshman, Y. E.; Chu, M.; Kim, T.; Kalmanson, O.; Farjah, M.; Kumar, M.; Lewis, W.; Geenen, D. L.; de Tombe, P.; Goldspink, P. H.; Solaro, R. J.; Samarel, A. M., Journal of Molecular and Cellular Cardiology
  • Contractile dysfunction in a mouse model expressing a heterozygous MYBPC3 mutation associated with hypertrophic cardiomyopathy Barefield, D.; Kumar, M.; de Tombe, P. P.; Sadayappan, S., American journal of physiology.Heart and circulatory physiology
  • Effects of a myofilament calcium sensitizer on left ventricular systolic and diastolic function in rats with volume overload heart failure Wilson, K.; Guggilam, A.; West, T. A.; Zhang, X.; Trask, A. J.; Cismowski, M. J.; de Tombe, P.; Sadayappan, S.; Lucchesi, P. A., American journal of physiology.Heart and circulatory physiology
  • Haploinsufficiency of MYBPC3 exacerbates the development of hypertrophic cardiomyopathy in heterozygous mice Barefield, D.; Kumar, M.; Gorham, J.; Seidman, J. G.; Seidman, C. E.; de Tombe, P. P.; Sadayappan, S., Journal of Molecular and Cellular Cardiology
  • Heart failure: a special issue van der Velden, J.; de Tombe, P. P., Pflugers Archiv : European journal of physiology
  • Myocardial infarction-induced N-terminal fragment of cardiac myosin-binding protein C (cMyBP-C) impairs myofilament function in human myocardium Witayavanitkul, N.; Ait Mou, Y.; Kuster, D. W.; Khairallah, R. J.; Sarkey, J.; Govindan, S.; Chen, X.; Ge, Y.; Rajan, S.; Wieczorek, D. F.; Irving, T.; Westfall, M. V.; de Tombe, P. P.; Sadayappan, S., The Journal of biological chemistry
  • Novel approaches to determine contractile function of the isolated adult zebrafish ventricular cardiac myocyte Dvornikov, A. V.; Dewan, S.; Alekhina, O. V.; Pickett, F. B.; de Tombe, P. P., The Journal of physiology
  • The Role of Dyadic Organization in Regulation of Sarcoplasmic Reticulum Ca(2+) Handling during Rest in Rabbit Ventricular Myocytes Bovo, E.; de Tombe, P. P.; Zima, A. V., Biophysical journal
  • Impact of titin isoform on length dependent activation and cross-bridge cycling kinetics in rat skeletal muscle Mateja, R. D.; Greaser, M. L.; de Tombe, P. P., Biochimica et biophysica acta
  • Mechanisms of Ca(2)+ handling in zebrafish ventricular myocytes Bovo, E.; Dvornikov, A. V.; Mazurek, S. R.; de Tombe, P. P.; Zima, A. V., Pflugers Archiv : European journal of physiology
  • New insights into the functional significance of the acidic region of the unique N-terminal extension of cardiac troponin I Henze, M.; Patrick, S. E.; Hinken, A.; Scruggs, S. B.; Goldspink, P.; de Tombe, P. P.; Kobayashi, M.; Ping, P.; Kobayashi, T.; Solaro, R. J., Biochimica et biophysica acta
  • Sex differences in health and disease: brain and heart connections--a special issue Wattanapermpool, J.; de Tombe, P. P.; Pak, T. R., Pflugers Archiv : European journal of physiology
  • Cardiac myosin binding protein-C: redefining its structure and function Sadayappan, S.; de Tombe, P. P., Biophysical reviews
  • Myofilament length-dependent activation develops within 5 ms in guinea-pig myocardium Mateja, R. D.; de Tombe, P. P., Biophysical journal
  • Pathogenic properties of the N-terminal region of cardiac myosin binding protein-C in vitro. Govindan, S.; Sarkey, J.; Ji, X.; Sundaresan, N. R.; Gupta, M. P.; de Tombe, P. P.; Sadayappan, S., Journal of Muscle Research & Cell Motility
  • The velocity of cardiac sarcomere shortening: mechanisms and implications de Tombe, P. P.; ter Keurs, H. E., JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY
  • Equal force recovery in dysferlin-deficient and wild-type muscles following saponin exposure. Zhao, P.; Xu, L.; Ait-Mou, Y.; de Tombe, P. P.; Han, R., Journal of Biomedicine & Biotechnology
  • Myosin head orientation: a structural determinant for the Frank-Starling relationship. Farman, G. P.; Gore, D.; Allen, E.; Schoenfelt, K.; Irving, T. C.; de Tombe, P. P., American Journal of Physiology - Heart & Circulatory Physiology
  • Nogo-A knockdown inhibits hypoxia/reoxygenation-induced activation of mitochondrial-dependent apoptosis in cardiomyocytes. Sarkey, J. P.; Chu, M.; McShane, M.; Bovo, E.; Mou, Y. A.; Zima, A. V.; de Tombe, P. P.; Kartje, G. L.; Martin, J. L., Journal of Molecular & Cellular Cardiology
  • Myofilament length dependent activation de Tombe, P. P.; Mateja, R. D.; Tachampa, K.; Mou, Y. A.; Farman, G. P.; Irving, T. C., Journal of Molecular & Cellular Cardiology
  • Ablation of ventricular myosin regulatory light chain phosphorylation in mice causes cardiac dysfunction in situ and affects neighboring myofilament protein phosphorylation. Scruggs, S. B.; Hinken, A. C.; Thawornkaiwong, A.; Robbins, J.; Walker, L. A.; de Tombe, P. P.; Geenen, D. L.; Buttrick, P. M.; Solaro, R. J., Journal of Biological Chemistry
  • Glass microneedles for force measurements: a finite-element analysis model. Ayittey, P. N.; Walker, J. S.; Rice, J. J.; de Tombe, P. P., Pflugers Archiv - European Journal of Physiology
  • Approximate model of cooperative activation and crossbridge cycling in cardiac muscle using ordinary differential equations. Rice, J. J.; Wang, F.; Bers, D. M.; de Tombe, P. P., Biophysical journal
  • Blebbistatin: use as inhibitor of muscle contraction. Farman, G. P.; Tachampa, K.; Mateja, R.; Cazorla, O.; Lacampagne, A.; de Tombe, P. P., Pflugers Archiv - European Journal of Physiology
  • Cardiac thin filament regulation Kobayashi, T.; Jin, L.; de Tombe, P. P., Pflugers Archiv - European Journal of Physiology
  • Delivery and visualization of proteins conjugated to quantum dots in cardiac myocytes. Koshman, Y. E.; Waters, S. B.; Walker, L. A.; Los, T.; de Tombe, P. P.; Goldspink, P. H.; Russell, B., Journal of Molecular & Cellular Cardiology
  • Differential contribution of cardiac sarcomeric proteins in the myofibrillar force response to stretch. Ait Mou, Y.; le Guennec, J. Y.; Mosca, E.; de Tombe, P. P.; Cazorla, O., Pflugers Archiv - European Journal of Physiology
  • Increased cross-bridge cycling kinetics after exchange of C-terminal truncated troponin I in skinned rat cardiac muscle. Tachampa, K.; Kobayashi, T.; Wang, H.; Martin, A. F.; Biesiadecki, B. J.; Solaro, R. J.; de Tombe, P. P., Journal of Biological Chemistry
  • Review focus series: sarcomeric proteins as key elements in integrated control of cardiac function. Solaro, R. J.; de Tombe, P. P., Cardiovascular research
  • Some rat: a very special rat with a rather special titin. Cazorla, O.; de Tombe, P. P., Journal of Molecular & Cellular Cardiology
  • Spatially-compressed cardiac myofilament models generate hysteresis that is not found in real muscle. Rice, J. J.; Tu, Y.; Poggesi, C.; De Tombe, P. P., Pacific Symposium on Biocomputing
  • Troponin phosphorylation and myofilament Ca2+-sensitivity in heart failure: increased or decreased?. Marston, S. B.; de Tombe, P. P., Journal of Molecular & Cellular Cardiology
  • Augmented protein kinase C-alpha-induced myofilament protein phosphorylation contributes to myofilament dysfunction in experimental congestive heart failure Belin, R. J.; Sumandea, M. P.; Allen, E. J.; Schoenfelt, K.; Wang, H.; Solaro, R. J.; de Tombe, P. P., Circulation research
  • Cardiac dysfunction and heart failure are associated with abnormalities in the subcellular distribution and amounts of oligomeric muscle LIM protein. Boateng, S. Y.; Belin, R. J.; Geenen, D. L.; Margulies, K. B.; Martin, J. L.; Hoshijima, M.; de Tombe, P. P.; Russell, B., American Journal of Physiology - Heart & Circulatory Physiology
  • Cardiac troponin I threonine 144: role in myofilament length dependent activation. Tachampa, K.; Wang, H.; Farman, G. P.; de Tombe, P. P., Circulation research
  • Development of contractile dysfunction in rat heart failure: hierarchy of cellular events. Daniels, M. C.; Naya, T.; Rundell, V. L.; de Tombe, P. P., American Journal of Physiology - Regulatory Integrative & Comparative Physiology
  • Frequency-dependent myofilament Ca2+ desensitization in failing rat myocardium. Lamberts, R. R.; Hamdani, N.; Soekhoe, T. W.; Boontje, N. M.; Zaremba, R.; Walker, L. A.; de Tombe, P. P.; van der Velden, J.; Stienen, G. J., Journal of Physiology
  • Impact of temperature on cross-bridge cycling kinetics in rat myocardium. de Tombe, P. P.; Stienen, G. J., Journal of Physiology
  • Interfilament spacing is preserved during sarcomere length isometric contractions in rat cardiac trabeculae. Farman, G. P.; Allen, E. J.; Gore, D.; Irving, T. C.; de Tombe, P. P., Biophysical journal
  • Myofilament calcium sensitivity does not affect cross-bridge activation-relaxation kinetics. de Tombe, P. P.; Belus, A.; Piroddi, N.; Scellini, B.; Walker, J. S.; Martin, A. F.; Tesi, C.; Poggesi, C., American Journal of Physiology - Regulatory Integrative & Comparative Physiology
  • p38-MAPK induced dephosphorylation of alpha-tropomyosin is associated with depression of myocardial sarcomeric tension and ATPase activity. Vahebi, S.; Ota, A.; Li, M.; Warren, C. M.; de Tombe, P. P.; Wang, Y.; Solaro, R. J., Circulation research
  • The troponin C G159D mutation blunts myofilament desensitization induced by troponin I Ser23/24 phosphorylation. Biesiadecki, B. J.; Kobayashi, T.; Walker, J. S.; John Solaro, R.; de Tombe, P. P., Circulation research