John Callaci
  • Associate Professor
  • Orthopaedic Surgery
  • Cell and Molecular Physiology
Research Keywords
  • Bone
  • Osteoporosis
Research Summary

Interests: Effects of Binge Alcohol Exposure on Skeletal Biology

My research is focused on the biochemical, biomechanical and molecular effects of alcohol on the skeleton. Previous studies performed in my laboratory using a binge model of alcohol consumption, identified that bone resorptive activity increases after acute binge alcohol exposure, which is at least partially responsible for bone loss observed after binge alcohol treatment. Alcohol-induced bone loss can be effectively blocked with anti-resorptive drugs, suggesting a possible therapeutic response to alcoholic osteopenia. We have also shown that binge alcohol exposure has an additive effect on bone loss caused by other insults to the skeleton such as ovariectomy-induced estrogen depletion. Research efforts identifying biomarkers of early alcohol-mediated bone damage in adolescent and adult ovariecotomized rodents using gene expression array technology allowed us to identify novel mechanisms underlying bone damage caused by alcohol and early markers of bone loss that can be used to identify patients at risk for osteoporosis years prior to conventional diagnostics. Our current research focuses on the mechanisms responsible for fracture non-union related to alcohol abuse. We have identified that alcohol deregulates key signaling pathways that control the fracture healing process. We are using this information to develop therapeutic approaches to treat patients with non healing fractures relayed to alcohol abuse and other causes such as diabetes.


Exogenous Activation of Wnt/beta-Catenin Signaling Attenuates Binge Alcohol-Induced Deficient Bone Fracture HealingLauing,K. L.; Sundaramurthy,S.; Nauer,R. K.; Callaci,J. J.Alcohol and Alcoholism (Oxford, Oxfordshire) 2014 ; ( ):

Ketorolac administration does not delay early fracture healing in a juvenile rat model: a pilot studyCappello,T.; Nuelle,J. A.; Katsantonis,N.; Nauer,R. K.; Lauing,K. L.; Jagodzinski,J. E.; Callaci,J. J.Journal of pediatric orthopedics 2013 ;33(4):415-421

The biomechanical and histologic effects of platelet-rich plasma on rat rotator cuff repairs.Beck,J.; Evans,D.; Tonino,P. M.; Yong,S.; Callaci,J. J.American Journal of Sports Medicine 2012 ;40(9):2037-2044

Mesenchymal stem cells facilitate fracture repair in an alcohol-induced impaired healing modelObermeyer,T. S.; Yonick,D.; Lauing,K.; Stock,S. R.; Nauer,R.; Strotman,P.; Shankar,R.; Gamelli,R.; Stover,M.; Callaci,J. J.Journal of orthopaedic trauma 2012 ;26(12):712-718

Binge alcohol exposure modulates rodent expression of biomarkers of the immunoinflammatory response to orthopaedic trauma.Sears,B. W.; Volkmer,D.; Yong,S.; Himes,R. D.; Lauing,K.; Morgan,M.; Stover,M. D.; Callaci,J. J.Journal of Bone & Joint Surgery - American Volume 2011 ;93(8):739-749

Alcohol exposure and mechanisms of tissue injury and repairJung,M. K.; Callaci,J. J.; Lauing,K. L.; Otis,J. S.; Radek,K. A.; Jones,M. K.; Kovacs,E. J.Alcoholism, Clinical and Experimental Research 2011 ;35(3):392-399

Long-term modulations in the vertebral transcriptome of adolescent-stage rats exposed to binge alcohol.Callaci,J. J.; Himes,R.; Lauing,K.; Roper,P.Alcohol & Alcoholism 2010 ;45(4):332-346

Effect of corticosteroids on the biomechanical strength of rat rotator cuff tendon.Mikolyzk,D. K.; Wei,A. S.; Tonino,P.; Marra,G.; Williams,D. A.; Himes,R. D.; Wezeman,F. H.; Callaci,J. J.Journal of Bone & Joint Surgery - American Volume 2009 ;91(5):1172-1180

Binge alcohol-induced bone damage is accompanied by differential expression of bone remodeling-related genes in rat vertebral bone.Callaci,J. J.; Himes,R.; Lauing,K.; Wezeman,F. H.; Brownson,K.Calcified tissue international 2009 ;84(6):474-484

Pathoanatomy and clinical correlates of the immunoinflammatory response following orthopaedic traumaSears,B. W.; Stover,M. D.; Callaci,J.Journal of the American Academy of Orthopaedic Surgeons 2009 ;17(4):255-265

Ethanol potentiates the acute fatty infiltration of liver caused by burn injury: prevention by insulin treatment.Emanuele,N. V.; Emanuele,M. A.; Morgan,M. O.; Sulo,D.; Yong,S.; Kovacs,E. J.; Himes,R. D.; Callaci,J. J.Journal of Burn Care & Research 2009 ;30(3):482-488

Distal tibiofibular bone-bridging in transtibial amputationPinzur,M. S.; Beck,J.; Himes,R.; Callaci,J.Journal of Bone & Joint Surgery, American Volume 2008 ;90(12):2682-2687

Binge alcohol treatment of adolescent rats followed by alcohol abstinence is associated with site-specific differences in bone loss and incomplete recovery of bone mass and strength.Lauing,K.; Himes,R.; Rachwalski,M.; Strotman,P.; Callaci,J. J.Alcohol 2008 ;42(8):649-656

Identification of novel bone-specific molecular targets of binge alcohol and ibandronate by transcriptome analysis.Himes,R.; Wezeman,F. H.; Callaci,J. J.Alcoholism: Clinical & Experimental Research 2008 ;32(7):1167-1180

Vitamin D and ibandronate prevent cancellous bone loss associated with binge alcohol treatment in male rats.Wezeman,F. H.; Juknelis,D.; Himes,R.; Callaci,J. J.Bone 2007 ;41(4):639-645

Binge alcohol treatment increases vertebral bone loss following ovariectomy: compensation by intermittent parathyroid hormone.Callaci,J. J.; Juknelis,D.; Patwardhan,A.; Wezeman,F. H.Alcoholism: Clinical & Experimental Research 2006 ;30(4):665-672

The effect of corticosteroid on collagen expression in injured rotator cuff tendon.Wei,A. S.; Callaci,J. J.; Juknelis,D.; Marra,G.; Tonino,P.; Freedman,K. B.; Wezeman,F. H.Journal of Bone & Joint Surgery - American Volume 2006 ;88(6):1331-1338

The effects of binge alcohol exposure on bone resorption and biomechanical and structural properties are offset by concurrent bisphosphonate treatment.Callaci,J. J.; Juknelis,D.; Patwardhan,A.; Sartori,M.; Frost,N.; Wezeman,F. H.Alcoholism: Clinical & Experimental Research 2004 ;28(1):182-191

Spine bone mineral density and vertebral body height are altered by alcohol consumption in growing male and female rats.Wezeman,F. H.; Juknelis,D.; Frost,N.; Callaci,J. J.Alcohol 2003 ;31(1-2):87-92