Alan Wolfe
  • Professor
  • Interim Director, Center for Biomedical Informatics
  • Microbiology and Immunology
Research Keywords
  • Molecular Microbiology
  • Genetics
  • Gene Regulation
  • Transcription

Urine is not sterile: use of enhanced urine culture techniques to detect resident bacterial flora in the adult female bladderHilt,E. E.; McKinley,K.; Pearce,M. M.; Rosenfeld,A. B.; Zilliox,M. J.; Mueller,E. R.; Brubaker,L.; Gai,X.; Wolfe,A. J.; Schreckenberger,P. C.Journal of clinical microbiology 2014 ;52(3):871-876

The metabolic enzyme AdhE controls the virulence of Escherichia coli O157:H7Beckham,K. S.; Connolly,J. P.; Ritchie,J. M.; Wang,D.; Gawthorne,J. A.; Tahoun,A.; Gally,D. L.; Burgess,K.; Burchmore,R. J.; Smith,B. O.; Beatson,S. A.; Byron,O.; Wolfe,A. J.; Douce,G. R.; Roe,A. J.Molecular microbiology 2014 ;93(1):199-211

The Female Urinary Microbiome: a Comparison of Women with and without Urgency Urinary IncontinencePearce,M. M.; Hilt,E. E.; Rosenfeld,A. B.; Zilliox,M. J.; Thomas-White,K.; Fok,C.; Kliethermes,S.; Schreckenberger,P. C.; Brubaker,L.; Gai,X.; Wolfe,A. J.mBio 2014 ;5(4):10.1128/mBio.01283-14

Urinary bacteria in adult women with urgency urinary incontinenceBrubaker,L.; Nager,C. W.; Richter,H. E.; Visco,A.; Nygaard,I.; Barber,M. D.; Schaffer,J.; Meikle,S.; Wallace,D.; Shibata,N.; Wolfe,A. J.International Urogynecology Journal 2014 ; ( ):

Structural, kinetic and proteomic characterization of acetyl phosphate-dependent bacterial protein acetylationKuhn,M. L.; Zemaitaitis,B.; Hu,L. I.; Sahu,A.; Sorensen,D.; Minasov,G.; Lima,B. P.; Scholle,M.; Mrksich,M.; Anderson,W. F.; Gibson,B. W.; Schilling,B.; Wolfe,A. J.PLoS ONE 2014 ;9(4):e94816

A tale of two machines: a review of the BLAST meeting, Tucson, AZ, 20-24 January 2013Josenhans,C.; Jung,K.; Rao,C. V.; Wolfe,A. J.Molecular microbiology 2014 ;91(1):6-25

Central metabolism controls transcription of a virulence gene regulator in Vibrio choleraeMinato,Y.; Fassio,S. R.; Wolfe,A. J.; Hase,C. C.Microbiology (Reading, England) 2013 ;159(Pt 4):792-802

Acetylation of the Response Regulator RcsB Controls Transcription from a Small RNA PromoterHu,L. I.; Chi,B. K.; Kuhn,M. L.; Filippova,E. V.; Walker-Peddakotla,A. J.; Basell,K.; Becher,D.; Anderson,W. F.; Antelmann,H.; Wolfe,A. J.Journal of Bacteriology 2013 ; ( ):

Day of surgery urine cultures identify urogynecologic patients at increased risk for postoperative urinary tract infectionFok,C. S.; McKinley,K.; Mueller,E. R.; Kenton,K.; Schreckenberger,P.; Wolfe,A.; Brubaker,L.The Journal of urology 2013 ;189(5):1721-1724

Evidence of uncultivated bacteria in the adult female bladder.Wolfe,A. J.; Toh,E.; Shibata,N.; Rong,R.; Kenton,K.; Fitzgerald,M.; Mueller,E. R.; Schreckenberger,P.; Dong,Q.; Nelson,D. E.; Brubaker,L.Journal of clinical microbiology 2012 ;50(4):1376-1383

Inhibition of acetyl phosphate-dependent transcription by an acetylatable lysine on RNA polymerase.Lima,B. P.; Thanh Huyen,T. T.; Basell,K.; Becher,D.; Antelmann,H.; Wolfe,A. J.Journal of Biological Chemistry 2012 ;287(38):32147-32160

Constitutive expression of the maltoporin LamB in the absence of OmpR damages the cell envelope.Reimann,S. A.; Wolfe,A. J.Journal of Bacteriology 2011 ;193(4):842-853

Bacterial protein acetylation: the dawning of a new ageHu,L. I.; Lima,B. P.; Wolfe,A. J.Molecular microbiology 2010 ;77(1):15-21

Physiologically relevant small phosphodonors link metabolism to signal transductionWolfe,A. J.Current opinion in microbiology 2010 ;13(2):204-209

Sighting the alien within: a new look at Bdellovibrio.Wolfe,A. J.Journal of Bacteriology 2010 ;192(24):6327-6328

A combination of assays reveals biomass differences in biofilms formed by Escherichia coli mutants.Sule,P.; Wadhawan,T.; Carr,N. J.; Horne,S. M.; Wolfe,A. J.; Pruss,B. M.Letters in applied microbiology 2009 ;49(3):299-304

A critical process controlled by MalT and OmpR is revealed through synthetic lethality.Reimann,S. A.; Wolfe,A. J.Journal of Bacteriology 2009 ;191(16):5320-5324

Optimized two-dimensional thin layer chromatography to monitor the intracellular concentration of acetyl phosphate and other small phosphorylated molecules.Keating,D. H.; Shulla,A.; Klein,A. H.; Wolfe,A. J.Biological Procedures Online 2008 ;10( ):36-46

Use of the BacTiter-GloTM microbial cell viability assay to study bacterial attachment.Sule,P.; Wadhawan,T.; Wolfe,A. J.Promega Notes 2008 ;99( ):19-21

Quorum sensing "flips" the acetate switchWolfe,A. J.Journal of Bacteriology 2008 ;190(17):5735-5737

Signal integration by the two-component signal transduction response regulator CpxR.Wolfe,A. J.; Parikh,N.; Lima,B. P.; Zemaitaitis,B.Journal of Bacteriology 2008 ;190(7):2314-2322

Get the message out: cyclic-Di-GMP regulates multiple levels of flagellum-based motilityWolfe,A. J.; Visick,K. L.Journal of Bacteriology 2008 ;190(2):463-475

The intracellular concentration of acetyl phosphate in Escherichia coli is sufficient for direct phosphorylation of two-component response regulators.Klein,A. H.; Shulla,A.; Reimann,S. A.; Keating,D. H.; Wolfe,A. J.Journal of Bacteriology 2007 ;189(15):5574-5581

The sugar phosphotransferase system of Vibrio fischeri inhibits both motility and bioluminescence.Visick,K. L.; O'Shea,T. M.; Klein,A. H.; Geszvain,K.; Wolfe,A. J.Journal of Bacteriology 2007 ;189(6):2571-2574

The multiple roles of CRP at the complex acs promoter depend on activation region 2 and IHF.Sclavi,B.; Beatty,C. M.; Thach,D. S.; Fredericks,C. E.; Buckle,M.; Wolfe,A. J.Molecular microbiology 2007 ;65(2):425-440

The two-component response regulator RcsB regulates type 1 piliation in Escherichia coli.Schwan,W. R.; Shibata,S.; Aizawa,S.; Wolfe,A. J.Journal of Bacteriology 2007 ;189(19):7159-7163

A complex transcription network controls the early stages of biofilm development by Escherichia coliPruss,B. M.; Besemann,C.; Denton,A.; Wolfe,A. J.Journal of Bacteriology 2006 ;188(11):3731-3739

Acetyl phosphate-sensitive regulation of flagellar biogenesis and capsular biosynthesis depends on the Rcs phosphorelay.Fredericks,C. E.; Shibata,S.; Aizawa,S.; Reimann,S. A.; Wolfe,A. J.Molecular microbiology 2006 ;61(3):734-747

Diguanylate cyclases control magnesium-dependent motility of Vibrio fischeri.O'Shea,T. M.; Klein,A. H.; Geszvain,K.; Wolfe,A. J.; Visick,K. L.Journal of Bacteriology 2006 ;188(23):8196-8205

The Escherichia coli K-12 NarL and NarP proteins insulate the nrf promoter from the effects of integration host factor.Browning,D. F.; Lee,D. J.; Wolfe,A. J.; Cole,J. A.; Busby,S. J.Journal of Bacteriology 2006 ;188(21):7449-7456

Integration of three signals at the Escherichia coli nrf promoter: a role for Fis protein in catabolite repression.Browning,D. F.; Grainger,D. C.; Beatty,C. M.; Wolfe,A. J.; Cole,J. A.; Busby,S. J.Molecular microbiology 2005 ;57(2):496-510

Magnesium promotes flagellation of Vibrio fischeri.O'Shea,T. M.; Deloney-Marino,C. R.; Shibata,S.; Aizawa,S.; Wolfe,A. J.; Visick,K. L.Journal of Bacteriology 2005 ;187(6):2058-2065

The acetate switchWolfe,A. J.Microbiology & Molecular Biology Reviews 2005 ;69(1):12-50

Glucose metabolism at high density growth of E. coli B and E. coli K: differences in metabolic pathways are responsible for efficient glucose utilization in E. coli B as determined by microarrays and Northern blot analysesPhue,J. N.; Noronha,S. B.; Hattacharyya,R.; Wolfe,A. J.; Shiloach,J.Biotechnology & Bioengineering 2005 ;90(7):805-820

Vibrio fischeri sigma54 controls motility, biofilm formation, luminescence, and colonization.Wolfe,A. J.; Millikan,D. S.; Campbell,J. M.; Visick,K. L.Applied & Environmental Microbiology 2004 ;70(4):2520-2524

Modulation of CRP-dependent transcription at the Escherichia coli acsP2 promoter by nucleoprotein complexes: anti-activation by the nucleoid proteins FIS and IHF.Browning,D. F.; Beatty,C. M.; Sanstad,E. A.; Gunn,K. E.; Busby,S. J.; Wolfe,A. J.Molecular microbiology 2004 ;51(1):241-254

Acetylation of the chemotaxis response regulator CheY by acetyl-CoA synthetase purified from Escherichia coli.Barak,R.; Prasad,K.; Shainskaya,A.; Wolfe,A. J.; Eisenbach,M.Journal of Molecular Biology 2004 ;342(2):383-401

Regulation at complex bacterial promoters: how bacteria use different promoter organizations to produce different regulatory outcomesBarnard,A.; Wolfe,A. J.; Busby,S.Current opinion in microbiology 2004 ;7(2):102-108

Chemoattraction of Vibrio fischeri to serine, nucleosides, and N-acetylneuraminic acid, a component of squid light-organ mucus.DeLoney-Marino,C. R.; Wolfe,A. J.; Visick,K. L.Applied & Environmental Microbiology 2003 ;69(12):7527-7530

Evidence that acetyl phosphate functions as a global signal during biofilm development.Wolfe,A. J.; Chang,D. E.; Walker,J. D.; Seitz-Partridge,J. E.; Vidaurri,M. D.; Lange,C. F.; Pruss,B. M.; Henk,M. C.; Larkin,J. C.; Conway,T.Molecular microbiology 2003 ;48(4):977-988

Cyclic AMP receptor protein-dependent activation of the Escherichia coli acsP2 promoter by a synergistic class III mechanism.Beatty,C. M.; Browning,D. F.; Busby,S. J.; Wolfe,A. J.Journal of Bacteriology 2003 ;185(17):5148-5157

Independent regulation of the divergent Escherichia coli nrfA and acsP1 promoters by a nucleoprotein assembly at a shared regulatory region.Browning,D. F.; Beatty,C. M.; Wolfe,A. J.; Cole,J. A.; Busby,S. J.Molecular microbiology 2002 ;43(3):687-701

Active site mutations in CheA, the signal-transducing protein kinase of the chemotaxis system in Escherichia coli.Hirschman,A.; Boukhvalova,M.; VanBruggen,R.; Wolfe,A. J.; Stewart,R. C.Biochemistry 2001 ;40(46):13876-13887

Regulation of acetyl coenzyme A synthetase in Escherichia coli.Kumari,S.; Beatty,C. M.; Browning,D. F.; Busby,S. J.; Simel,E. J.; Hovel-Miner,G.; Wolfe,A. J.Journal of Bacteriology 2000 ;182(15):4173-4179

sigma(70) is the principal sigma factor responsible for transcription of acs, which encodes acetyl coenzyme A synthetase in Escherichia coli.Kumari,S.; Simel,E. J.; Wolfe,A. J.Journal of Bacteriology 2000 ;182(2):551-554

Effects of mutations in acetate metabolism on high-cell-density growth of Escherichia coliContiero,J.; Beatty,C.; Kumari,S.; DeSanti,C. L.; Strohl,W. R.; Wolfe,A. J.Journal of industrial microbiology & biotechnology 2000 ;24(6):421-430

Global regulatory mutations in csrA and rpoS cause severe central carbon stress in Escherichia coli in the presence of acetate.Wei,B.; Shin,S.; LaPorte,D.; Wolfe,A. J.; Romeo,T.Journal of Bacteriology 2000 ;182(6):1632-1640

Polar clustering of the chemoreceptor complex in Escherichia coli occurs in the absence of complete CheA function.Skidmore,J. M.; Ellefson,D. D.; McNamara,B. P.; Couto,M. M.; Wolfe,A. J.; Maddock,J. R.Journal of Bacteriology 2000 ;182(4):967-973

  • California Institute of Technology, Research
  • Harvard University, Research
  • History and Social Studies Books
  • Music
  • Soccer